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Lastly, through a genome-wide CRISPR loss-of-function screen, we confirmed that the effects of LCS3 are related to antioxidant response and found that inactivation of NADPH quinone oxidoreductase 1 (NQO1) impairs ROS production and confers resistance to LCS3

Competing Interests The authors declare that they have no competing interests Materials and Methods Plasmid construction Amino acids mutations to switch Rex-domain specificity from NAD to NADP were selected based on a previous screen [15]

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